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The role of reactive oxygen species and hemeoxygenase-1 expression in the cytotoxicity, cell cycle alteration and apoptosis of dental pulp cells induced by BisGMA.
- Source :
-
Biomaterials [Biomaterials] 2010 Nov; Vol. 31 (32), pp. 8164-71. Date of Electronic Publication: 2010 Jul 31. - Publication Year :
- 2010
-
Abstract
- Biocompatibility of dentin bonding agents (DBAs) and resin composite is important to preserve the pulp vitality after operative restoration. Bisphenol-glycidyl-methacrylate (BisGMA) is one common monomer adding into DBAs and resin. In this study, we found that exposure of human dental pulp cells to BisGMA (>0.1 mM) led to cytotoxicity, G2/M cell cycle arrest and apoptosis as analyzed by propidium iodide (PI) and PI/annexin V dual fluorescent flow cytometry. These events were associated with a decline of cdc2, cdc25C and cyclinB1 expression at both mRNA and protein levels. BisGMA also induced the expression of hemeoxygenase-1 (HO-1), an oxidative stress responsive gene, in pulp cells. Catalase could prevent the BisGMA-induced alteration of cell cycle-related genes (cdc2, cdc25C, cyclinB1) and HO-1 expression in dental pulp cells. Interestingly, Zn-protoporphyrin (2.5-5 microM), a HO inhibitor, enhanced the BisGMA-induced reactive oxygen species (ROS) production and cytotoxicity. These results suggest that exposure to higher concentrations of BisGMA may stimulate ROS production, cell cycle arrest, apoptosis and cell death. Inducing the expression of HO-1 in dental pulp cells by BisGMA is mediated by ROS production and important to protect dental pulp against the toxicity by monomers present in composite resin and DBAs.<br /> (Copyright (c) 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Catalase metabolism
Cells, Cultured
Gene Expression Regulation drug effects
Humans
Porphyrins metabolism
Apoptosis drug effects
Bisphenol A-Glycidyl Methacrylate adverse effects
Cell Cycle drug effects
Dental Pulp cytology
Dentin-Bonding Agents adverse effects
Heme Oxygenase-1 genetics
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 31
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 20673999
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2010.07.049