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Expression of inflammation-associated genes in circulating leukocytes collected from horses with gastrointestinal tract disease.
- Source :
-
American journal of veterinary research [Am J Vet Res] 2010 Aug; Vol. 71 (8), pp. 915-24. - Publication Year :
- 2010
-
Abstract
- Objective: To investigate whether expression of inflammation-associated genes in leukocytes from horses with gastrointestinal tract (GIT) diseases correlated with the type of disease and outcome.<br />Animals: 10 healthy horses and 50 horses with GIT disease.<br />Procedures: A blood sample was collected from each healthy horse or horse with GIT disease (during admission to the hospital). Leukocytes were isolated, diluted to a standard concentration, and frozen until RNA extraction. Expression of 14 genes associated with inflammation was quantified by use of a real-time quantitative reverse transcription PCR assay. Results were grouped by GIT disease type and disease outcome for comparison.<br />Results: Horses with GIT disease had colic of unknown etiology (n = 8 horses), GIT inflammation or strangulation (19), or nonstrangulating GIT obstruction (23). Among the 45 horses receiving treatment, 38 were discharged from the hospital, and 7 died or were euthanized. Compared with healthy horses, horses with colic of unknown etiology had similar gene expression. Significant differences in expression of the interleukin-8, leukocyte-selectin molecule, matrix metalloproteinase-9, platelet-selectin molecule, mitochondrial superoxide dismutase, Toll-like receptor 4, and tumor necrosis factor-A genes were detected between healthy horses and horses with GIT disease. Significant differences in expression of the interleukin-1 receptor antagonist, interleukin-8, leukocyte-selectin molecule, matrix metalloproteinase-9, platelet-selectin molecule, mitochondrial superoxide dismutase, Toll-like receptor 4, and tumor necrosis factor-A genes were detected among healthy horses and horses grouped by disease outcome.<br />Conclusions and Clinical Relevance: Inflammatory gene expression in leukocytes of horses with GIT disease appeared to be related to disease pathogenesis and prognosis.
- Subjects :
- Animals
Colic blood
Colic genetics
Colic physiopathology
Colic veterinary
DNA genetics
DNA isolation & purification
Euthanasia
Gastrointestinal Diseases genetics
Gastrointestinal Diseases mortality
Gastrointestinal Diseases physiopathology
Horse Diseases mortality
Horse Diseases physiopathology
Horses
Inflammation blood
Inflammation genetics
RNA genetics
RNA isolation & purification
Reference Values
Reverse Transcriptase Polymerase Chain Reaction
Gastrointestinal Diseases veterinary
Horse Diseases blood
Horse Diseases genetics
Inflammation veterinary
Leukocytes physiology
Toll-Like Receptor 4 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9645
- Volume :
- 71
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 20673091
- Full Text :
- https://doi.org/10.2460/ajvr.71.8.915