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Induction of apoptosis by gene transfer of human TRAIL mediated by arginine-rich intracellular delivery peptides.
- Source :
-
Anticancer research [Anticancer Res] 2010 Jun; Vol. 30 (6), pp. 2193-202. - Publication Year :
- 2010
-
Abstract
- Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of the tumor necrosis factor (TNF) family, has shown potent and high selective antitumor activity as a promising therapy for cancer. We have developed an arginine-rich intracellular delivery (AID) peptide-mediated system for nontoxic and efficient gene transfer in cells.<br />Materials and Methods: To evaluate antitumor activity and therapeutic potential of TRAIL gene, a bifunctional expression plasmid was constructed encoding the secretory signal peptide of human immunoglobulin kappa (Ig kappa) light chain, the extracellular portion (amino acids 95-281) of human TRAIL and the humanized green fluorescent protein (GFP).<br />Results: We demonstrated that AID peptides were able to effectively deliver TRAIL gene into human lung carcinoma A549 cells. Soluble TRAIL-GFP protein purified from media after gene delivery was further evaluated regarding selective induction of apoptosis in cells.<br />Conclusion: AID peptide-mediated DNA transfer provides a potential and convenient tool in nonviral gene therapy.
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 30
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 20651369