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Phenotypic morphology and the expression of cytoskeletal markers during long-term differentiation of human SH-SY5Y neuroblastoma cells.

Authors :
Hartley CL
Johnston HB
Nicol S
Chan KM
Baines AJ
Anderton BH
Thomas SM
Source :
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 1996 Oct; Vol. 10 (5), pp. 539-50.
Publication Year :
1996

Abstract

A simple method for differentiating human-derived SH-SY5Y neuroblastoma cells to provide a stable, mature, neuronal morphology is described. SH-SY5Y cells can be induced to differentiate terminally with retinoic acid in medium with low levels of serum. The morphological differentiation of the parental cell line SK-N-SH was compared with that seen in the SH-SY5Y cells. Changes in the cytoskeleton of SH-SY5Y cells indicated that differentiation proceeds continuously over the 1-month period studied after initiating differentiation. Immunoblot analysis demonstrated increased expression of the high molecular weight neurofilament polypeptide NF-H, the microtubule-associated protein tau, and the synaptic vesicle-associated protein synapsin I, indicating that an increasingly mature, neuronal phenotype was being expressed. The cultures were not dependent on retinoic acid for continued survival. SH-SY5Y cultures differentiated over extended periods should provide a good in vitro model for studying the neurotoxic potential of compounds and mechanisms of toxicity, particularly in longer-term or multiple exposure studies, for example on cytoskeletal function, where acute toxicity is not the aspect of interest.

Details

Language :
English
ISSN :
0887-2333
Volume :
10
Issue :
5
Database :
MEDLINE
Journal :
Toxicology in vitro : an international journal published in association with BIBRA
Publication Type :
Academic Journal
Accession number :
20650235
Full Text :
https://doi.org/10.1016/s0887-2333(96)00041-0