Utility of pandemic H1N1 2009 influenza virus recombinant hemagglutinin protein-based enzyme-linked immunosorbent assay for serosurveillance.

Authors :: Arankalle VA
Virkar RG
Tandale BV
Ingle NB
Source :: Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2010 Sep; Vol. 17 (9), pp. 1481-3. Date of Electronic Publication: 2010 Jul 14.
Publication Year :: 2010
Abstract: An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG antibodies against the pandemic H1N1 2009 influenza A virus, employing a recombinant hemagglutinin protein of the virus, was compared to the hemagglutination inhibition (HI) test using 783 serum samples. The results showed a concordance of 98.4%, suggesting the utility of the ELISA in serosurveillance. Two hundred sixty-nine (100%) serum samples with an HI titer of > or =20 were ELISA reactive.

Subjects :: Enzyme-Linked Immunosorbent Assay methods
Hemagglutination Inhibition Tests
Humans
Immunoglobulin G blood
Recombinant Proteins genetics
Sensitivity and Specificity
Antibodies, Viral blood
Antigens, Viral genetics
Hemagglutinins, Viral genetics
Influenza A Virus, H1N1 Subtype immunology
Influenza, Human immunology
Influenza, Human virology
Virology methods

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