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Tegumental surface changes in adult Fasciola hepatica in response to treatment in vivo with triclabendazole in the sheep host.
- Source :
-
Veterinary parasitology [Vet Parasitol] 2010 Sep 20; Vol. 172 (3-4), pp. 238-48. Date of Electronic Publication: 2010 May 16. - Publication Year :
- 2010
-
Abstract
- Eight indoor-reared crossbred sheep with no pre-exposure to Fasciola hepatica were infected by oral gavage with 200 metacercarial cysts of the triclabendazole (TCBZ)-susceptible Cullompton isolate of F. hepatica. At 12 weeks post-infection, sheep were dosed with 10mg/kg triclabendazole. Two sheep per time period were euthanized at 48h, 72h and 96h post-treatment. Two control sheep were euthanized alongside the 96h triclabendazole-treated sheep. Flukes were recovered from each of the sheeps' liver and, if present, from the gall bladder, and they were processed for scanning electron microscopy (SEM). Flukes recovered 48h post-treatment were active. Disruption to the tegument took the form of swelling, widespread blebbing and some loss of the tegument covering the spines. By 72h post-treatment, all flukes recovered were dead and a number were recovered from the gall bladder. Typically, the posterior end of the flukes was elongated and in this region the tegumental syncytium had sloughed away. Tegumental loss was more widespread on flukes recovered from the gall bladder. At 96h post-treatment, only one fluke was recovered from the liver and three from the gall bladder. All the flukes were dead and they were totally devoid of tegumental syncytium.
- Subjects :
- Animals
Fasciola hepatica ultrastructure
Fascioliasis drug therapy
Fascioliasis parasitology
Gallbladder parasitology
Liver parasitology
Microscopy, Electron, Scanning
Random Allocation
Sheep
Triclabendazole
Benzimidazoles pharmacology
Benzimidazoles therapeutic use
Fasciola hepatica drug effects
Fascioliasis veterinary
Sheep Diseases drug therapy
Sheep Diseases parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2550
- Volume :
- 172
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 20627418
- Full Text :
- https://doi.org/10.1016/j.vetpar.2010.05.012