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Collateral capillary arterialization following arteriolar ligation in murine skeletal muscle.
- Source :
-
Microcirculation (New York, N.Y. : 1994) [Microcirculation] 2010 Jul; Vol. 17 (5), pp. 333-47. - Publication Year :
- 2010
-
Abstract
- Objective: Chronic and acute ischemic diseases-peripheral artery disease, coronary artery disease, stroke-result in tissue damage unless blood flow is maintained or restored in a timely manner. Mice of different strains recover from arteriolar ligation (by increasing collateral blood flow) at different speeds. We quantify the spatio-temporal patterns of microvascular network remodeling following arteriolar ligation in different mouse strains to better understand inter-individual variability.<br />Methods: Whole-muscle spinotrapezius microvascular networks of mouse strains C57Bl/6, Balb/c and CD1 were imaged using confocal microscopy following ligation of feeding arterioles.<br />Results: Baseline arteriolar structures of C57Bl/6 and Balb/c mice feature heavily ramified arcades and unconnected dendritic trees, respectively. This network angioarchitecture identifies ischemia-protected and ischemia-vulnerable tissues; unlike C57Bl/6, downstream capillary perfusion in Balb/c spinotrapezius is lost following ligation. Perfusion recovery requires arterialization (expansion and investment of mural cells) of a subset of capillaries forming a new low-resistance collateral pathway between arteriolar trees. Outbred CD1 exhibit either Balb/c-like or C57Bl/6-like spinotrapezius angioarchitecture, predictive of response to arteriolar ligation.<br />Conclusions: This collateral capillary arterialization process may explain the reported longer time required for blood flow recovery in Balb/c hindlimb ischemia, as low-resistance blood flow pathways along capillary conduits must be formed ("arterialization") before reperfusion.
- Subjects :
- Animals
Arterioles growth & development
Arterioles pathology
Capillaries growth & development
Capillaries pathology
Disease Models, Animal
Female
Ligation
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Reperfusion
Species Specificity
Collateral Circulation
Ischemia pathology
Microcirculation
Muscle, Skeletal blood supply
Subjects
Details
- Language :
- English
- ISSN :
- 1549-8719
- Volume :
- 17
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Microcirculation (New York, N.Y. : 1994)
- Publication Type :
- Academic Journal
- Accession number :
- 20618691
- Full Text :
- https://doi.org/10.1111/j.1549-8719.2010.00034.x