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Inflammatory mediators generated at the site of inoculation of Loxosceles gaucho spider venom.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2010 Nov; Vol. 56 (6), pp. 972-9. Date of Electronic Publication: 2010 Jul 06. - Publication Year :
- 2010
-
Abstract
- Patients bitten by Loxosceles spiders generally manifest marked local inflammatory reaction and dermonecrosis. This report evaluated edema formation, leukocyte infiltration and release of inflammatory mediators at the injection site of Loxosceles gaucho venom. BALB/c mice were i.d. injected with venom and thereafter paws were disrupted and homogenized to obtain differential counts of migrated cells, as well to assay the levels of cytokines, chemokines and lipid mediators. Increased footpad thickness was detected as soon as 30 min after venom injection, and 24h later was similar to that of the control group. Loxosceles venom mildly augmented the recruitment of leukocytes to the footpad in comparison with PBS-injected mice. Moreover, it stimulated the release of IL-6, MCP-1 and KC at 2 and 24h after venom injection. In addition, higher levels of PGE(2) were detected 30 min after venom injection in comparison with control group. However, the venom failed to increase levels of IL-1 beta, TNF-alpha, TXB(2) and LTB(4). Our results demonstrate that L. gaucho venom evokes an early complex inflammatory reaction, stimulating the secretion of pro-inflammatory cytokines and lipid mediators (PGE(2)), and recruiting leukocytes to the footpad which contribute to the local reaction induced by L. gaucho venom.<br /> ((c) 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Biomarkers metabolism
Disease Models, Animal
Edema chemically induced
Edema metabolism
Edema pathology
Hindlimb
Inflammation metabolism
Inflammation pathology
Leukocyte Count
Male
Mice
Mice, Inbred BALB C
Neutrophil Infiltration drug effects
Neutrophil Infiltration physiology
Phosphoric Diester Hydrolases immunology
Spider Bites immunology
Spider Bites pathology
Spider Venoms immunology
Inflammation chemically induced
Inflammation Mediators metabolism
Phosphoric Diester Hydrolases toxicity
Serine Endopeptidases
Spider Bites metabolism
Spider Venoms toxicity
Spiders physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3150
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 20615425
- Full Text :
- https://doi.org/10.1016/j.toxicon.2010.06.022