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Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka.
- Source :
-
Gene expression patterns : GEP [Gene Expr Patterns] 2010 Sep; Vol. 10 (6), pp. 283-9. Date of Electronic Publication: 2010 Jun 17. - Publication Year :
- 2010
-
Abstract
- In the teleost fish, medaka (Oryzias latipes), the sex is genetically determined at the time of fertilization. The males are heterogametic with XY chromosome composition, while females are of XX chromosome composition. The male sexual differentiation is initiated in XY embryos of medaka by the sex-determining gene Dmy. In this study, we have cloned the gonadal soma derived factor (Gsdf) from medaka and characterized its expression pattern during the initiation of morphological testicular differentiation. By real-time PCR, an XY-specific up-regulation was detected in the expression levels of Gsdf in the whole embryos of medaka at 6days post fertilization (dpf), coincident with the initiation of testicular differentiation in the XY gonads. Whole mount and section in situ hybridizations reaffirmed that Gsdf was expressed exclusively in primordial gonads of only the genetic males at 6dpf. Conversely, the expression of Gsdf was found to be very weak in the XX gonads during embryogenesis. Importantly, Gsdf and Dmy were found to be co-localized in the same somatic cells in the XY gonads. When the XY embryos were treated with estradiol-17beta, in order to reverse their phenotypic sex, a decline was observed in the expression of Gsdf in these embryos by real-time PCR.<br /> (Copyright 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Embryo, Nonmammalian
Female
Fish Proteins metabolism
Gene Expression Regulation, Developmental
Germ Cells metabolism
Germ Cells physiology
Gonads embryology
Gonads metabolism
Male
Molecular Sequence Data
Oryzias metabolism
Phylogeny
Sequence Homology, Amino Acid
Testis embryology
Time Factors
Fish Proteins genetics
Oryzias embryology
Oryzias genetics
Sex Differentiation genetics
Testis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7298
- Volume :
- 10
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Gene expression patterns : GEP
- Publication Type :
- Academic Journal
- Accession number :
- 20601164
- Full Text :
- https://doi.org/10.1016/j.gep.2010.06.005