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Genetic control of immune responses to influenza A matrix 2 protein (M2).
- Source :
-
Vaccine [Vaccine] 2010 Aug 16; Vol. 28 (36), pp. 5817-27. Date of Electronic Publication: 2010 Jul 16. - Publication Year :
- 2010
-
Abstract
- Vaccines should protect genetically diverse populations. Therefore we tested the candidate "universal" influenza A matrix protein 2 (M2) vaccine in multiple mouse strains. Mice were primed with M2 DNA and boosted with M2 recombinant adenovirus (rAd). C57BL/6 (B6) mice developed no antibody or T-cell response to M2, while BALB/c responded strongly. CBA responses were intermediate. Both MHC and background genes influenced responsiveness. To improve low responses we immunized with adjuvanted peptide-carrier conjugates, or co-immunized with nucleoprotein (NP), which can augment T-cell help. The conjugate vaccine enhanced some outcomes but not others. Co-immunizing with NP improved outcomes over either NP or M2 immunizations alone. These results have implications for vaccination of genetically diverse populations.
- Subjects :
- Animals
Antibodies, Viral blood
Antibody Formation
Female
Immunity, Cellular
Immunoglobulin G blood
Major Histocompatibility Complex
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Orthomyxoviridae Infections immunology
Orthomyxoviridae Infections prevention & control
T-Lymphocytes immunology
Vaccines, Conjugate immunology
Vaccines, DNA immunology
Influenza A virus immunology
Influenza Vaccines immunology
Orthomyxoviridae Infections genetics
Viral Matrix Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 28
- Issue :
- 36
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 20600476
- Full Text :
- https://doi.org/10.1016/j.vaccine.2010.06.069