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Role of transient receptor potential A1 in gastric nociception.
- Source :
-
Digestion [Digestion] 2010; Vol. 82 (3), pp. 150-5. Date of Electronic Publication: 2010 Jun 25. - Publication Year :
- 2010
-
Abstract
- Afferent fibers innervating the gastrointestinal tract have major roles in consciously evoked sensations including pain. However, little is known about the molecules involved in mechanonociception from the upper gastrointestinal tract. We recently reported that activation of extracellular signal-regulated kinase 1/2 (ERK1/2), a member of the mitogen-activated protein kinase cascade in primary afferent neurons, was induced by noxious gastric distention in the rat, and that the activation of ERK1/2 in dorsal root ganglion (DRG) neurons can be implicated in acute visceral pain. Transient receptor potential (TRP) A1, a member of the TRP family of cation channels, was expressed in both DRG and nodose ganglion (NG) neurons innervating the stomach and in nerve fibers in the gastric wall. TRPA1 was coexpressed with ERK1/2 in gastric primary afferent neurons, and attenuation of TRPA1 activation using antisense peptides and a specific blocker led to suppression of both ERK1/2 activation and visceromotor responses. TRPA1 also significantly colocalized with substance P (SP) and calcitonin gene-related peptide (CGRP) in the thoracolumbar DRG, NG and stomach. These data indicate that SP and CGRP may also be released by TRPA1 activation in primary afferent neurons to elicit neurogenic inflammation and promote visceral hyperalgesia.<br /> (Copyright 2010 S. Karger AG, Basel.)
- Subjects :
- Animals
Butadienes
Denervation
Enzyme Activation
Enzyme Inhibitors
Extracellular Signal-Regulated MAP Kinases metabolism
Ganglia, Spinal metabolism
Gastric Dilatation physiopathology
Male
Nitriles
Nodose Ganglion metabolism
Oligodeoxyribonucleotides, Antisense
Rats
Rats, Sprague-Dawley
Stomach innervation
Stomach physiopathology
TRPA1 Cation Channel
Gastric Dilatation metabolism
Gastric Mucosa metabolism
Neurogenic Inflammation metabolism
Nociceptors metabolism
TRPC Cation Channels metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1421-9867
- Volume :
- 82
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Digestion
- Publication Type :
- Academic Journal
- Accession number :
- 20588026
- Full Text :
- https://doi.org/10.1159/000310836