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Cell adhesion to unnatural ligands mediated by a bifunctional protein.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2010 Jul 21; Vol. 132 (28), pp. 9733-7. - Publication Year :
- 2010
-
Abstract
- This paper describes a molecular strategy to restore adhesion of cells to surfaces that otherwise do not present ligands that can mediate adhesion. The approach is based on a carbonic anhydrase fusion protein that binds benzenesulfonamides and that also includes the RGD peptide motif that can bind to cell-surface integrin adhesion receptors. In this way, the fusion protein can bind to a monolayer that presents the benzenesulfonamide ligand, thereby positioning the RGD peptide at the surface, where it can mediate the adhesion and spreading of cells. This strategy may provide a general method for promoting the adhesion of cells to non-natural surfaces or to defective biological matrices.
Details
- Language :
- English
- ISSN :
- 1520-5126
- Volume :
- 132
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 20583796
- Full Text :
- https://doi.org/10.1021/ja1016188