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Importance of dynamic culture for evaluating osteoblast activity on dense silicon-substituted hydroxyapatite.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2010 Oct 15; Vol. 80 (2), pp. 138-44. Date of Electronic Publication: 2010 Jun 01. - Publication Year :
- 2010
-
Abstract
- This paper reports an investigation on human osteoblast-like cells (SaOs-2) seeded onto pure hydroxyapatite (HA) and silicon-substituted HA (SiHA) tablets under static and dynamic culture conditions. The biological characterizations were conducted in classical static conditions in multi-wells plates, and in a perfusion bioreactor that permits continuous circulation of culture medium at 2 mL/h. The morphology, proliferation and differentiation of osteoblastic cells were examined for the two types of samples in the both culture conditions after 1, 3 and 8 days. Under dynamic conditions, cells cultured on SiHA surfaces showed a faster adhesion process and the formation of longer and thinner focal adhesions than in static conditions. The number of cells grown onto both ceramic surfaces was higher in dynamic conditions when compared with static conditions. Moreover, a higher activity of alkaline phosphatase was found for cells seeded under dynamic conditions. Our findings suggest that the application of perfusion culture system on cells cultured on dense substrates is valuable for predicting in vivo behaviour of cells on biomaterials.<br /> (Copyright (c) 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Alkaline Phosphatase metabolism
Biocompatible Materials adverse effects
Cell Adhesion drug effects
Cells, Cultured
Durapatite adverse effects
Enzyme Activation drug effects
Humans
Osteoblasts metabolism
Biocompatible Materials chemistry
Biocompatible Materials pharmacology
Durapatite chemistry
Durapatite pharmacology
Osteoblasts cytology
Osteoblasts drug effects
Silicon chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 80
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 20579858
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2010.05.040