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Poly(A)-binding protein 1 partially relocalizes to the nucleus during herpes simplex virus type 1 infection in an ICP27-independent manner and does not inhibit virus replication.
- Source :
-
Journal of virology [J Virol] 2010 Sep; Vol. 84 (17), pp. 8539-48. Date of Electronic Publication: 2010 Jun 23. - Publication Year :
- 2010
-
Abstract
- Infection of cells by herpes simplex virus type 1 (HSV-1) triggers host cell shutoff whereby mRNAs are degraded and cellular protein synthesis is diminished. However, virus protein translation continues because the translational apparatus in HSV-infected cells is maintained in an active state. Surprisingly, poly(A)-binding protein 1 (PABP1), a predominantly cytoplasmic protein that is required for efficient translation initiation, is partially relocated to the nucleus during HSV-1 infection. This relocalization occurred in a time-dependent manner with respect to virus infection. Since HSV-1 infection causes cell stress, we examined other cell stress inducers and found that oxidative stress similarly relocated PABP1. An examination of stress-induced kinases revealed similarities in HSV-1 infection and oxidative stress activation of JNK and p38 mitogen-activated protein (MAP) kinases. Importantly, PABP relocalization in infection was found to be independent of the viral protein ICP27. The depletion of PABP1 by small interfering RNA (siRNA) knockdown had no significant effect on viral replication or the expression of selected virus late proteins, suggesting that reduced levels of cytoplasmic PABP1 are tolerated during infection.
- Subjects :
- Animals
Carrier Proteins
Cell Nucleus genetics
Chlorocebus aethiops
Gene Expression Regulation, Viral
HeLa Cells
Herpes Simplex virology
Herpesvirus 1, Human genetics
Humans
Immediate-Early Proteins genetics
Oxidative Stress
Poly(A)-Binding Protein I genetics
Protein Transport
Vero Cells
Cell Nucleus metabolism
Herpes Simplex metabolism
Herpesvirus 1, Human physiology
Immediate-Early Proteins metabolism
Poly(A)-Binding Protein I metabolism
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 84
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 20573819
- Full Text :
- https://doi.org/10.1128/JVI.00668-10