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Integrin expression profiling identifies integrin alpha5 and beta1 as prognostic factors in early stage non-small cell lung cancer.
- Source :
-
Molecular cancer [Mol Cancer] 2010 Jun 17; Vol. 9, pp. 152. Date of Electronic Publication: 2010 Jun 17. - Publication Year :
- 2010
-
Abstract
- Background: Selection of early stage non-small cell lung cancer patients with a high risk of recurrence is warranted in order to select patients who will benefit from adjuvant treatment strategies. We evaluated the prognostic value of integrin expression profiles in a retrospective study on frozen primary tumors of 68 patients with early stage non-small cell lung cancer.<br />Methods: A retrospective study was performed on frozen primary tumors of 68 early stage non-small cell lung cancer patients with a follow up of at least 10 years. From all tumor tissues, RNA was isolated and reverse transcribed into cDNA. qPCR was used to generate mRNA expression profiles including integrins alpha1, 2, 3, 4, 5, 6, 7, 11, and V as well as integrins beta1, 3, 4, 5, 6, and 8.<br />Results: The expression levels of integrins alpha5, beta1 and beta3 predicted overall survival and disease free survival in early stage NSCLC patients. There was no association between integrin expression and lymph node metastases. Comparison between the histological subtypes revealed a distinct integrin signature for squamous cell carcinoma while the profiles of adenocarcinoma and large cell carcinoma were largely the same.<br />Conclusion: Integrin expression in NSCLC is important for the development and behavior of the tumor and influences the survival of the patient. Determining the integrin expression profile might serve as a tool in predicting the prognosis of individual patients.
- Subjects :
- Adult
Aged
Aged, 80 and over
Biomarkers, Tumor analysis
Carcinoma, Non-Small-Cell Lung metabolism
Carcinoma, Non-Small-Cell Lung mortality
Disease-Free Survival
Female
Gene Expression
Humans
Integrin alphaV genetics
Integrin beta1 genetics
Kaplan-Meier Estimate
Lung Neoplasms metabolism
Lung Neoplasms mortality
Male
Middle Aged
Prognosis
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Carcinoma, Non-Small-Cell Lung genetics
Gene Expression Profiling
Integrin alphaV biosynthesis
Integrin beta1 biosynthesis
Lung Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4598
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular cancer
- Publication Type :
- Academic Journal
- Accession number :
- 20565758
- Full Text :
- https://doi.org/10.1186/1476-4598-9-152