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Isolation, characterization and cDNA sequencing of acrosin from turkey spermatozoa.
- Source :
-
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2010 Sep; Vol. 157 (1), pp. 127-36. Date of Electronic Publication: 2010 Jun 01. - Publication Year :
- 2010
-
Abstract
- Acrosin (EC 3.4.21.10) is serine proteinase localized in the sperm acrosome and considered to play an essential role in fertilization. In contrast to mammalian, there are only limited data concerning avian acrosin, mostly focused on the characterization of mature enzyme. In the present study, acrosin was isolated from turkey spermatozoa using gel filtration in the presence of 4 M urea at acidic pH. N-terminal Edman sequencing allowed the identification of the first 26 N-terminal amino acids: VVGGTEALHG SWPWIVSIQNPRFAGT. This sequence was used to construct primers and obtain a cDNA sequence from the testis. The amino acid sequence deduced from the cDNA shows that turkey acrosin is initially synthesized as prepro-protein with 19-residue signal peptide. This signal sequence is followed by a 327-residue sequence corresponding to the acrosin zymogen. Turkey proacrosin does not contain a proline-rich segment at the C-terminal portion. Mature turkey acrosin is a two-chain molecule consisting of light and heavy chains and was found to be glycoprotein. The proacrosin/acrosin system exists in turkey spermatozoa and this system can be activated similarly to that of mammals. The high value of association constant strongly suggests that acrosin activity in turkeys can be controlled by a seminal plasma Kazal inhibitor under physiological conditions.<br /> (2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Acrosin chemistry
Acrosin metabolism
Amidohydrolases metabolism
Amino Acid Sequence
Animals
Base Sequence
Chemical Phenomena
Chromatography, Gel
Cloning, Molecular
Enzyme Precursors metabolism
Gelatinases metabolism
Humans
Male
Molecular Sequence Data
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trypsin Inhibitor, Kazal Pancreatic metabolism
Acrosin genetics
Acrosin isolation & purification
DNA, Complementary genetics
Spermatozoa enzymology
Turkeys genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1107
- Volume :
- 157
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 20561916
- Full Text :
- https://doi.org/10.1016/j.cbpb.2010.05.011