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Translational comparison of HPV58 long and short L1 mRNAs in yeast (Saccharomyces cerevisiae) cell-free system.

Authors :
Wang X
Liu J
Zhao WM
Zhao KN
Source :
Journal of bioscience and bioengineering [J Biosci Bioeng] 2010 Jul; Vol. 110 (1), pp. 58-65. Date of Electronic Publication: 2010 Feb 20.
Publication Year :
2010

Abstract

Recently, we developed a yeast cell-free system for translation of human papillomavirus (HPV) 58 short L1 mRNA and VLP assembly in vitro. In the present study, we examined the translation efficiency of HPV58 long and short L1 mRNAs in our established system. Production of HPV 58 long and short L1 proteins was significantly dependent on the amounts of the L1 mRNAs used and the time length of translation reaction. Compared with the long L1 mRNA, the short L1 mRNA consistently exhibited higher translation efficiency in all the experiments conducted. Supplementation of exogenous amino acids significantly enhanced translation of the long L1 mRNA in this system. Furthermore, a single amino acid leucine can be rate-limiting for translation of the long L1 mRNA in this system. Electron microscopy demonstrated that HPV 58 virus-like particles (VLPs) were assembled from both long and short L1 capsid proteins in the yeast cell-free system.<br /> (2009 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1347-4421
Volume :
110
Issue :
1
Database :
MEDLINE
Journal :
Journal of bioscience and bioengineering
Publication Type :
Academic Journal
Accession number :
20541117
Full Text :
https://doi.org/10.1016/j.jbiosc.2010.01.011