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Calcium requirements for the proliferation of cells infected with a temperature-sensitive mutant of Rous sarcoma virus.
- Source :
-
Cancer research [Cancer Res] 1978 May; Vol. 38 (5), pp. 1237-40. - Publication Year :
- 1978
-
Abstract
- Between 32 degrees and 39 degrees, uninfected normal rat kidney (NRK) cells proliferated minimally, or not at all, in medium containing 0 to 0.01 mM extracellular free calcium. Transformation of NRK cells by wild-type avian sarcoma virus B77 enabled them to proliferate equally rapidly at all temperatures in high (1.25 mM)- or low (0.001 mM-calcium medium. Transformation by the temperature-sensitive LA-23 mutant of Rous sarcoma virus also enabled NRK cells to proliferate in low (0.001 mM)-calcium medium at 32 degrees or 37 degrees. However, the LA-23 NRK cells could not proliferate in low (0.001 mM)-calcium medium at a nonpermissive temperature such as 39 degrees, which prevents expression of other aspects of the transformed phenotype.
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 38
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 205359