Back to Search
Start Over
T cell and APC dynamics in situ control the outcome of vaccination.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2010 Jul 01; Vol. 185 (1), pp. 239-52. Date of Electronic Publication: 2010 Jun 07. - Publication Year :
- 2010
-
Abstract
- The factors controlling the progression of an immune response to generation of protective memory are poorly understood. We compared the in situ and ex vivo characteristics of CD8 T cells responding to different forms of the same immunogen. Immunization with live Listeria monocytogenes, irradiated L. monocytogenes (IRL), or heat-killed L. monocytogenes (HKL) induced rapid activation of CD8 T cells. However, only IRL and live L. monocytogenes inoculation induced sustained proliferation and supported memory development. Gene and protein expression analysis revealed that the three forms of immunization led to three distinct transcriptional and translational programs. Prior to cell division, CD8 T cell-dendritic cell clusters formed in the spleen after live L. monocytogenes and IRL but not after HKL immunization. Furthermore, HKL immunization induced rapid remodeling of splenic architecture, including loss of marginal zone macrophages, which resulted in impaired bacterial clearance. These results identify initial characteristics of a protective T cell response that have implications for the development of more effective vaccination strategies.
- Subjects :
- Animals
Antigen-Presenting Cells microbiology
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes microbiology
CD8-Positive T-Lymphocytes transplantation
Cell Communication genetics
Cell Communication immunology
Dendritic Cells immunology
Dendritic Cells metabolism
Gene Expression Profiling
Immunization Schedule
Listeriosis microbiology
Lymphocyte Activation genetics
Lymphocyte Activation immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
T-Lymphocyte Subsets microbiology
T-Lymphocyte Subsets transplantation
Treatment Outcome
Vaccines, Attenuated administration & dosage
Vaccines, Attenuated immunology
Vaccines, Inactivated administration & dosage
Vaccines, Inactivated immunology
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic immunology
Antigen-Presenting Cells immunology
Bacterial Vaccines administration & dosage
Bacterial Vaccines immunology
Listeriosis immunology
Listeriosis prevention & control
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 185
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 20530268
- Full Text :
- https://doi.org/10.4049/jimmunol.0901047