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Localization of the two tropomyosin-binding sites of troponin T.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2010 Aug 15; Vol. 500 (2), pp. 144-50. Date of Electronic Publication: 2010 Jun 08. - Publication Year :
- 2010
-
Abstract
- Troponin T (TnT) binds to tropomyosin (Tm) to anchor the troponin complex in the thin filament, and it thus serves as a vital link in the Ca(2+) regulation of striated muscle contraction. Pioneer work three decades ago determined that the T1 and T2 chymotryptic fragments of TnT each contains a Tm-binding site. A more precise localization of the two Tm-binding sites of TnT remains to be determined. In the present study, we tested serial deletion constructs of TnT and carried out monoclonal antibody competition experiments to show that the T1 region Tm-binding site involves mainly a 39 amino acids segment in the N-terminal portion of the conserved middle region of TnT. We further employed another set of TnT fragments to locate the T2 region Tm-binding site to a segment of 25 amino acids near the beginning of the T2 fragment. The localization of the two Tm-binding sites of TnT provided new information for the structure-function relationship of TnT and the anchoring of troponin complex on muscle thin filament.<br /> (2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Antibodies, Monoclonal
Binding Sites
Binding, Competitive
Conserved Sequence
Enzyme-Linked Immunosorbent Assay
In Vitro Techniques
Mice
Models, Molecular
Molecular Sequence Data
Muscle, Skeletal metabolism
Peptide Fragments chemistry
Peptide Fragments genetics
Peptide Fragments metabolism
Protein Binding
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Sequence Homology, Amino Acid
Tropomyosin chemistry
Troponin T chemistry
Troponin T genetics
Troponin T immunology
Tropomyosin metabolism
Troponin T metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0384
- Volume :
- 500
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 20529660
- Full Text :
- https://doi.org/10.1016/j.abb.2010.06.001