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Ontogeny of D-mannose transport and metabolism in rat small intestine.
- Source :
-
The Journal of membrane biology [J Membr Biol] 2010 Jun; Vol. 235 (2), pp. 101-8. Date of Electronic Publication: 2010 Jun 04. - Publication Year :
- 2010
-
Abstract
- Oral mannose therapy is used to treat congenital disorders of glycosylation caused by a deficiency in phosphomannose isomerase. The segmental distribution and ontogenic regulation of D-mannose transport, phosphomannose isomerase, and phosphomannose mutase is investigated in the small intestine of fetuses, newborn, suckling, 1-month-old, and adult rats. The small intestine transports D-mannose by both Na(+)-dependent and Na(+)-independent transport mechanisms. The activities of both systems normalized to intestinal weight peak at birth and thereafter they decreased. In all the ages tested, the activity of the Na(+)-independent mechanism was higher than that of the Na(+)/mannose transport system. At birth, the Na(+)-independent D-mannose transport in the ileum was significantly higher than that in jejunum. Phosphomannose isomerase activity and mRNA levels increased at 1 month, and the values in the ileum were lower than in jejunum. Phosphomannose mutase activity in jejunum increased during the early stages of life, and it decreased at 1 month old, as does the amount of mannose incorporated into glycoproteins, whereas in the ileum, they were not affected by age. The phosphomannose isomerase/phosphomannose mutase activity ratio decreased at birth and during the suckling period, and increased at 1 month old. In conclusion, intestinal D-mannose transport activity and metabolism were affected by ontogeny and intestinal segment.
- Subjects :
- Animals
Biological Transport genetics
Gene Expression Regulation, Developmental genetics
Gene Expression Regulation, Developmental physiology
Mannose-6-Phosphate Isomerase genetics
Mannose-6-Phosphate Isomerase metabolism
Models, Biological
Phosphotransferases (Phosphomutases) genetics
Phosphotransferases (Phosphomutases) metabolism
Polymerase Chain Reaction
Rats
Biological Transport physiology
Intestine, Small metabolism
Mannose metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1424
- Volume :
- 235
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of membrane biology
- Publication Type :
- Academic Journal
- Accession number :
- 20523973
- Full Text :
- https://doi.org/10.1007/s00232-010-9259-0