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Bacillus nanhaiensis sp. nov., isolated from an oyster.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2011 Apr; Vol. 61 (Pt 4), pp. 888-893. Date of Electronic Publication: 2010 May 21. - Publication Year :
- 2011
-
Abstract
- A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, catalase-positive, oxidase-negative, endospore-forming, motile, rod-shaped, aerobic bacterium, designated strain JSM 082006(T), was isolated from an oyster collected from Naozhou Island in the South China Sea. The isolate grew in 0-18 % (w/v) NaCl (optimum, 0.5-4.0 %), at pH 6.0-10.5 (optimum, pH 8.0) and at 15-45 °C (optimum, 30 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and C(16 : 0). Strain JSM 082006(T) contained MK-7 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 082006(T) should be assigned to the genus Bacillus and that it was most closely related to the type strains of Bacillus barbaricus (sequence similarity 99.1 %) and Bacillus arsenicus (97.5 %), followed by those of Bacillus rigui (96.6 %) and Bacillus solisalsi (96.1 %). Phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data support the view that strain JSM 082006(T) represents a novel species of the genus Bacillus, for which the name Bacillus nanhaiensis sp. nov. is proposed; the type strain is JSM 082006(T) ( = DSM 23009(T) = KCTC 13712(T)).
- Subjects :
- Aerobiosis
Alkalies metabolism
Animals
Bacillus genetics
Bacillus physiology
Base Composition
Catalase metabolism
Cell Wall chemistry
China
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Diaminopimelic Acid analysis
Fatty Acids analysis
Molecular Sequence Data
Nucleic Acid Hybridization
Oxidoreductases metabolism
Peptidoglycan chemistry
Phospholipids analysis
Phylogeny
Quinones analysis
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Sodium Chloride metabolism
Spores, Bacterial cytology
Temperature
Bacillus classification
Bacillus isolation & purification
Ostreidae microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 61
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 20495032
- Full Text :
- https://doi.org/10.1099/ijs.0.022889-0