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[Protein micro-crystallography with a new micro-beam beamline].
- Source :
-
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan [Yakugaku Zasshi] 2010 May; Vol. 130 (5), pp. 641-8. - Publication Year :
- 2010
-
Abstract
- Recently, researchers' demands for high-quality data collection from protein micro-crystals, which would usually result from crystallization difficulty, have been remarkably growing. In order to collect efficient diffraction data for structure determination from micro-crystals, an expert data collection system providing high signal-to-noise ratio should be essential. A small sized and highly brilliant X-ray beam with size of a few micrometers has been proved to achieve protein micro-crystallography by both increasing reflection intensities and reducing background scattering from sample environments. Two micro-beam beamlines at SPring-8 and KEK-PF are currently under construction for Targeted Proteins Research Program by MEXT of Japan. At SPring-8, a new undulator beamline for protein micro-crystallography, named RIKEN Targeted Proteins Beamline (BL32XU), will start operation in May, 2010. The beam size at sample position of BL32XU will correspond to 1 x 1 microm(2) with the photon flux density over 6 x 10(10) photons/s/microm(2). Furthermore, research and developments of indispensable components to achieve protein micro-crystallography with micro-beam, such as high-precision diffractometer, automated micro-crystal handling, advanced data collection system to suppress radiation damage and so on, are progressing. The present status of the research and development for protein micro-crystallography will be presented.
Details
- Language :
- Japanese
- ISSN :
- 0031-6903
- Volume :
- 130
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
- Publication Type :
- Academic Journal
- Accession number :
- 20460858
- Full Text :
- https://doi.org/10.1248/yakushi.130.641