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Discs large 5 is required for polarization of citron kinase in mitotic neural precursors.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2010 May 15; Vol. 9 (10), pp. 1990-7. Date of Electronic Publication: 2010 May 15. - Publication Year :
- 2010
-
Abstract
- Citron kinase (CitK), a protein essential to neurogenic cell division in the central nervous system, is highly polarized in neural progenitors. The mechanisms that polarize CitK to cellular domains that line the ventricular surface of neuroepithelium are currently not known. Here we report that Discs large 5 (Dlg5), a member of the MAGUK family, is an interactor of CitK required for CitK polarization. The CitK-Dlg5 interaction was first revealed in a protein array screen of proteins containing PDZ domains, and then subsequently confirmed by co-immunoprecipitation. Moreover, in Dlg5 (-/-) mice CitK fails to polarize in mitotic neuronal precursors. In addition, the total number of mitotic progenitors and the ratio of ventricular to abventricular mitotic progenitors in developing neocortex are significantly decreased in Dlg5 (-/-) embryos. Dlg5 is therefore required to maintain the polarization of a protein essential to neurogenic cytokinesis, and plays a role in localizing cell divisions to the surface of the lateral ventricles in embryonic brain.
- Subjects :
- Animals
Brain embryology
Brain metabolism
Cell Division physiology
Cells, Cultured
Guanylate Kinases genetics
Immunohistochemistry
Immunoprecipitation
Intracellular Signaling Peptides and Proteins genetics
Lateral Ventricles embryology
Lateral Ventricles metabolism
Membrane Proteins genetics
Mice
Mice, Knockout
Mitosis genetics
Mitosis physiology
Protein Binding
Protein Serine-Threonine Kinases genetics
Rats
Guanylate Kinases metabolism
Intracellular Signaling Peptides and Proteins metabolism
Membrane Proteins metabolism
Neurons cytology
Neurons metabolism
Protein Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 20436275
- Full Text :
- https://doi.org/10.4161/cc.9.10.11730