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Neuropathogenic and non-neuropathogenic variants of equine herpesvirus 1 in France.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2010 Oct 26; Vol. 145 (3-4), pp. 329-33. Date of Electronic Publication: 2010 Apr 09. - Publication Year :
- 2010
-
Abstract
- Equine herpesvirus 1 (EHV-1) is a common pathogen of the horse which may induce mild respiratory distress, abortion, neonatal death and neurological disease. A single nucleotide polymorphism in the EHV-1 DNA polymerase (ORF30 A(2254) to G(2254)) has been associated with clinical signs of Equine herpes myeloencephalopathy (EHM). The aim of this work was to analyze the ORF30 genomic region among a panel of EHV-1 DNA extract in order to estimate the prevalence of the EHV-1 neuropathogenic genotype in France. Samples coming from cases associated with EHM, horses with respiratory symptoms and aborted mares, each obtained between 2002 and 2009, were investigated. DNA was directly extracted from biological samples and allelic discrimination was performed using real-time PCR. Thirty of the 125 analysed horses (24%) presented the G(2254) genotype of ORF 30. Among them, 7/16 were provided by EHM cases, 1/24 by respiratory cases and 22/85 by abortion cases. Concerning EHM, the 7 G(2254) genotype of ORF30 were all isolated in 2009 during two outbreaks where mortality was observed. Regarding the 22 G(2254) genotype of ORF 30, 17 were identified in foetuses on which EHV-1 was detected by PCR, without any certainty of viral implication in the abortion. These findings clearly suggest that other factors need to be considered for a better understanding of the impact of DNA polymerase genotype upon EHV-1 neuropathogenic phenotype.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
DNA, Viral chemistry
DNA, Viral genetics
DNA-Directed DNA Polymerase chemistry
DNA-Directed DNA Polymerase genetics
France epidemiology
Genotype
Herpesviridae Infections epidemiology
Herpesviridae Infections virology
Herpesvirus 1, Equid classification
Herpesvirus 1, Equid genetics
Horse Diseases epidemiology
Horses
Nervous System Diseases epidemiology
Nervous System Diseases virology
Open Reading Frames genetics
Polymerase Chain Reaction veterinary
Prevalence
Genetic Variation genetics
Herpesviridae Infections veterinary
Herpesvirus 1, Equid isolation & purification
Horse Diseases virology
Nervous System Diseases veterinary
Polymorphism, Single Nucleotide genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 145
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 20427133
- Full Text :
- https://doi.org/10.1016/j.vetmic.2010.03.031