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Flow cytometric analysis for the mechanism of the new antineoplastic agent temozolomide in glioma cells.
- Source :
-
Brain tumor pathology [Brain Tumor Pathol] 2010 Apr; Vol. 27 (1), pp. 7-15. Date of Electronic Publication: 2010 Apr 28. - Publication Year :
- 2010
-
Abstract
- Temozolomide (TMZ) has been accepted as a standard antitumor drug for glioma worldwide. Regarding its mechanism of action, there are quite a few analyses. In the present study, we investigated the cell-killing effect and mechanism of action of TMZ with flow cytometry using glioblastoma cell lines. Each cell line was divided into three groups: a control group, a low-dose TMZ group, and a high-dose TMZ group. On day 1, TMZ was added to each cell line. Then, we counted the numbers of cells on days 2, 3, 4, and 5; in U87MG, we counted the number of cells on days 8 and 9. Simultaneously, we performed flow cytometric analysis with single- and double-staining methods. Although results varied slightly depending on the cell line, with flow cytometric analysis we identified the G(0)G(1)-, S-phase block on days 2 through 4, at the beginning of TMZ administration. After that we identified the deviation of the G(2)M block over days 3 to 5. Dominant morphological changes observed in U87MG were confined to the nuclei, with positive TUNEL staining. Early S-phase block and then a G(2)M block were observed; consecutively, we could analyze these blocks with a double-staining method more clearly. The flow cytometric method is very effective in the analysis of the antitumor mechanism of each agent. On the basis of our analysis, more effective combined chemotherapy may be expected.
- Subjects :
- Animals
Antineoplastic Agents metabolism
Cell Line, Tumor
Dacarbazine metabolism
Dacarbazine pharmacology
Dose-Response Relationship, Drug
Drug Resistance, Neoplasm
Flow Cytometry methods
Glioma metabolism
Humans
Rats
Staining and Labeling
Temozolomide
Antineoplastic Agents pharmacology
Dacarbazine analogs & derivatives
Glioma pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1861-387X
- Volume :
- 27
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Brain tumor pathology
- Publication Type :
- Academic Journal
- Accession number :
- 20425042
- Full Text :
- https://doi.org/10.1007/s10014-009-0259-7