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Monitoring of loss of heterozygosity in serum microsatellite DNA among patients with gastrointestinal stromal tumors indicates tumor recurrence.
- Source :
-
The Journal of surgical research [J Surg Res] 2011 Jul; Vol. 169 (1), pp. 31-5. Date of Electronic Publication: 2010 Jan 27. - Publication Year :
- 2011
-
Abstract
- Background: Patients with gastrointestinal stromal tumors (GIST) harbor increased levels of circulating tumor DNA in their peripheral blood. In the current study, the aim was to investigate whether the frequency of loss of heterozygosity (LOH) on cell-free DNA in blood may reflect tumor stage and recurrent disease of these patients.<br />Materials and Methods: Serum DNA and follow-up samples of 92 patients suffering from recurrent GIST were analyzed by a PCR-based fluorescence microsatellite analysis using a panel of 12 polymorphic markers. The data were correlated with established risk factors, and patients were followed-up over 4 y.<br />Results: Microsatellite analysis demonstrated a positive LOH score on cell-free DNA of 30/92 patients. A significant correlation with recurrence in CT imaging showed that a positive LOH score (n ≥ 2) was detected in 58% (11/19) of patients with recurrent disease (P = 0.030, χ(2) test), but only in 25% of patients were clinically free of recurrence. No prognostic significance of a positive LOH score was observed after a median observation time of 48 mo.<br />Conclusion: Our findings show that LOH on circulating serum DNA correlates with the tumor status and is a frequent event in GIST patients with recurrent disease.<br /> (Published by Elsevier Inc.)
- Subjects :
- Biomarkers, Tumor blood
Disease Progression
Female
Follow-Up Studies
Gastrointestinal Stromal Tumors diagnostic imaging
Gastrointestinal Stromal Tumors genetics
Humans
Longitudinal Studies
Male
Middle Aged
Neoplasm Recurrence, Local blood
Neoplasm Recurrence, Local genetics
Predictive Value of Tests
Prognosis
Retrospective Studies
Tomography, X-Ray Computed
DNA, Neoplasm blood
DNA, Neoplasm genetics
Gastrointestinal Stromal Tumors blood
Loss of Heterozygosity genetics
Microsatellite Repeats genetics
Neoplasm Recurrence, Local diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8673
- Volume :
- 169
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 20417938
- Full Text :
- https://doi.org/10.1016/j.jss.2009.12.032