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Early phenotypical diagnoses in Trembler-J mice model.
- Source :
-
Journal of neuroscience methods [J Neurosci Methods] 2010 Jun 30; Vol. 190 (1), pp. 14-9. Date of Electronic Publication: 2010 Apr 21. - Publication Year :
- 2010
-
Abstract
- Pmp-22 mutant mice (Trembler-J: B6.D2-Pmp22<Tr-J>/J), are used as a model to study Charcot-Marie-Tooth type 1A (CMT1A). The identification of individual genotypes is a routine in the management of the Tr(J) colony. The earliest phenotypic manifestation of the pmp-22 mutation is just about 20th postnatal days, when pups begin to tremble. In this study, a rapid and simple diagnostic method was developed by modifying the Tail Suspension Test (MTST) to determine the difference between the Tr(J) and the wild-type mice phenotype. The animal behavioral phenotypes generated during the test were consistent with the specific genotype of each animal. The MTST allowed us to infer the heterozygous genotype in early postnatal stages, at 11 days after birth. The motor impairment of Tr(J) mice was also analyzed by a Fixed Bar Test (FBT), which revealed the disease evolution according to age. The main advantages of MTST are its objectivity, simplicity, and from the viewpoint of animal welfare, it is a non-invasive technique that combined with his rapidity show its very well applicability for use from an early age in these mice.<br /> (Copyright 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Age Factors
Animals
Charcot-Marie-Tooth Disease genetics
Disease Models, Animal
Dyskinesias diagnosis
Dyskinesias genetics
Early Diagnosis
Female
Genetic Carrier Screening methods
Heterozygote
Male
Mice
Mice, Mutant Strains
Myelin Proteins genetics
Sequence Analysis, DNA
Tail
Charcot-Marie-Tooth Disease diagnosis
Disability Evaluation
Hindlimb Suspension methods
Phenotype
Subjects
Details
- Language :
- English
- ISSN :
- 1872-678X
- Volume :
- 190
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neuroscience methods
- Publication Type :
- Academic Journal
- Accession number :
- 20416338
- Full Text :
- https://doi.org/10.1016/j.jneumeth.2010.04.010