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Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22.
- Source :
-
Journal of periodontal research [J Periodontal Res] 2010 Aug; Vol. 45 (4), pp. 488-95. Date of Electronic Publication: 2010 Apr 19. - Publication Year :
- 2010
-
Abstract
- Background and Objective: Recent epidemiological studies have shown a correlation between periodontitis and hyperlipidemia. We have found high levels of oxidized low-density lipoprotein (OxLDL) in the gingival crevicular fluid of dental patients. In the present study, we tried to examine the possible role of OxLDL in periodontal inflammation in vitro.<br />Material and Methods: Cells of the human gingival epithelial cell line Ca9-22 were cultured in media containing OxLDL, and the amounts of interleukin-8 (IL-8) and prostaglandin E(2) (PGE(2)) produced were measured using ELISAs.<br />Results: Production of IL-8 by Ca9-22 cells was significantly increased when the cells were treated with OxLDL, but not with native LDL or acetylated LDL. Production of PGE(2) by Ca9-22 cells was enhanced by co-incubation with OxLDL and interleukin-1 beta (IL-1 beta). Scavenger receptor inhibitors, fucoidan and dextran sulfate, inhibited the OxLDL-induced IL-8 and PGE(2) production in the presence of IL-1 beta. The p(38) MAPK inhibitors SB203580 and SB202190 and the ERK inhibitor PD98059 inhibited the OxLDL-induced IL-8 production. Among oxidized lipids and chemically modified LDL, 7-ketocholesterol enhanced IL-8 production.<br />Conclusion: This is the first report to show that OxLDL enhances IL-8 production in epithelial cells.
- Subjects :
- Cell Line, Tumor
Chemokine CCL2 analysis
Cholesterol 7-alpha-Hydroxylase antagonists & inhibitors
Dextran Sulfate pharmacology
Dinoprostone analysis
Enzyme Inhibitors pharmacology
Epithelial Cells drug effects
Flavonoids pharmacology
Fucose pharmacology
Gingiva cytology
Humans
Imidazoles pharmacology
Interleukin-1beta analysis
Interleukin-1beta pharmacology
Interleukin-8 analysis
Interleukin-8 antagonists & inhibitors
Ketocholesterols pharmacology
Lipoproteins, LDL antagonists & inhibitors
Mitogen-Activated Protein Kinases antagonists & inhibitors
Oxidation-Reduction
Periodontitis metabolism
Polysaccharides pharmacology
Pyridines pharmacology
Receptors, Scavenger antagonists & inhibitors
Sulfuric Acid Esters pharmacology
p38 Mitogen-Activated Protein Kinases antagonists & inhibitors
Gingiva drug effects
Interleukin-8 drug effects
Lipoproteins, LDL pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0765
- Volume :
- 45
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of periodontal research
- Publication Type :
- Academic Journal
- Accession number :
- 20412422
- Full Text :
- https://doi.org/10.1111/j.1600-0765.2009.01263.x