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Anaplasma phagocytophilum from Rodents and Sheep, China.
- Source :
-
Emerging infectious diseases [Emerg Infect Dis] 2010 May; Vol. 16 (5), pp. 764-8. - Publication Year :
- 2010
-
Abstract
- To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
- Subjects :
- Anaplasma phagocytophilum cytology
Anaplasma phagocytophilum genetics
Animals
China epidemiology
Citrate (si)-Synthase analysis
Citrate (si)-Synthase genetics
Cricetinae
DNA, Bacterial genetics
DNA, Bacterial isolation & purification
Ehrlichiosis epidemiology
Ehrlichiosis microbiology
HL-60 Cells
Heat-Shock Proteins analysis
Heat-Shock Proteins genetics
Humans
Membrane Proteins analysis
Membrane Proteins genetics
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S analysis
RNA, Ribosomal, 16S genetics
Rodent Diseases epidemiology
Sequence Analysis, DNA
Sheep
Sheep Diseases epidemiology
Anaplasma phagocytophilum isolation & purification
Ehrlichiosis veterinary
Rodent Diseases microbiology
Sheep Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1080-6059
- Volume :
- 16
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Emerging infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 20409364
- Full Text :
- https://doi.org/10.3201/eid1605.021293