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Investigation of ractopamine molecularly imprinted stir bar sorptive extraction and its application for trace analysis of beta2-agonists in complex samples.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2010 May 28; Vol. 1217 (22), pp. 3612-8. Date of Electronic Publication: 2010 Mar 30. - Publication Year :
- 2010
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Abstract
- In this paper, a novel molecularly imprinted polymer (MIP) coated stir bar with ractopamine as template by glass capillary filling with magnetic core as substrate was prepared reproducibly. The ractopamine MIP coating was homogeneous and porous with the average thickness of 20.6 microm. The extraction apparatus for the stir bar was improved to avoid coating loss. The MIP-coated stir bar showed better extraction capacity and good selectivity than that of non-imprinted polymer (NIP) coated stir bar to ractopamine and its analogues. The extraction capacities of ractopamine, isoxsuprine, clenbuterol and fenoterol for MIP-coated stir bar were 3.3, 3.1, 2.8 and 2.4 times as much as that of the NIP coated stir bar, respectively. The MIP-coated stir bars could be used at least 40 times without apparent damage and kept in dried air for 8 months without reduce of extraction ability. A method for the determination of beta(2)-agonists in complex samples by MIP-coated stir bar sorptive extraction coupled with high-performance liquid chromatography (HPLC) was developed. The linear ranges were 0.5-40 microg/L for ractopamine and 1.0-40 microg/L for isoxsuprine and clenbuterol. The detection limits were within the range of 0.10-0.21 microg/L. The method was successfully applied to the analysis of beta(2)-agonists in spiked pork, liver and feed samples with the recoveries of 83.7-92.3%, 80.5-90.2% and 73.6-86.2%, respectively. The RSDs was within 2.9-8.1%. The method is very suitable for the determination of trace beta(2)-agonists in pork, liver and feed samples.<br /> (Copyright 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Adrenergic beta-Agonists chemistry
Animal Feed analysis
Animals
Clenbuterol analysis
Clenbuterol chemistry
Fenoterol analysis
Fenoterol chemistry
Food Analysis methods
Isoxsuprine analysis
Isoxsuprine chemistry
Linear Models
Liver chemistry
Meat analysis
Microscopy, Electron, Scanning
Phenethylamines chemistry
Reproducibility of Results
Sensitivity and Specificity
Spectroscopy, Fourier Transform Infrared
Swine
Thermogravimetry
Adrenergic beta-Agonists analysis
Chemical Fractionation methods
Chromatography, High Pressure Liquid methods
Molecular Imprinting methods
Phenethylamines analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1217
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 20399437
- Full Text :
- https://doi.org/10.1016/j.chroma.2010.03.046