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A novel flow cytometric antibody panel for distinguishing Burkitt lymphoma from CD10+ diffuse large B-cell lymphoma.
- Source :
-
American journal of clinical pathology [Am J Clin Pathol] 2010 May; Vol. 133 (5), pp. 718-26. - Publication Year :
- 2010
-
Abstract
- Rapid and accurate differential diagnosis between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL) is imperative because their treatment differs. Recent studies have characterized several antigens differentially expressed in these 2 types of lymphoma. Our goal was to determine whether use of these markers would aid in the differential diagnosis of BL vs CD10+ DLBCL by flow cytometric immunophenotyping (FCI). Twenty-three cases of CD10+ B-cell lymphomas with available cryopreserved samples were identified (13 BL and 10 CD10+ DLBCL). Multiparameter FCI was performed using the following antibodies: CD18, CD20, CD43, CD44, and CD54 and isotype controls. Expression of CD44 and CD54 was detected at a significantly lower level in BL compared with CD10+ DLBCL (P = .001 and P = .01, respectively). There was not a significant difference in expression of CD18 and CD43. Our data show that expression of CD44 and CD54 differs significantly between BL and CD10+ DLBCL.
- Subjects :
- Adult
Aged
Biomarkers, Tumor metabolism
Burkitt Lymphoma immunology
Burkitt Lymphoma metabolism
Child, Preschool
Diagnosis, Differential
Female
Humans
Immunophenotyping
Lymphoma, Large B-Cell, Diffuse immunology
Lymphoma, Large B-Cell, Diffuse metabolism
Male
Middle Aged
Antibodies, Monoclonal immunology
Antibodies, Neoplasm immunology
Burkitt Lymphoma pathology
Flow Cytometry methods
Lymphoma, Large B-Cell, Diffuse pathology
Neprilysin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1943-7722
- Volume :
- 133
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 20395518
- Full Text :
- https://doi.org/10.1309/AJCP0XQDGKFR0HTW