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CD5+ B cells from individuals with systemic lupus erythematosus express granzyme B.
- Source :
-
European journal of immunology [Eur J Immunol] 2010 Jul; Vol. 40 (7), pp. 2060-9. - Publication Year :
- 2010
-
Abstract
- Recently, we reported that IL-21 induces granzyme B (GzmB) and GzmB-dependent apoptosis in malignant CD5(+) B cells from patients with chronic lymphocytic leukemia. Several autoimmune diseases (AD) are associated with enhanced frequencies of CD5(+) B cells. Since AD are also associated with elevated IL-21 and GzmB levels, we postulated a link between CD5(+) B cells, IL-21 and GzmB. Here, we demonstrate that IL-21 and GzmB serum levels are highly correlated in subjects with systemic lupus erythematosus (SLE) and that freshly isolated CD5(+) SLE B cells constitutively express GzmB. IL-21 directly induced GzmB expression and secretion by CD5(+) B cells from several AD and from cord blood in vitro, and the simultaneous presence of BCR stimulation strongly enhanced this process. Furthermore, IL-21 suppressed both viability and expansion of CD5(+) B cells from SLE individuals. In summary, our study may explain the elevated levels of IL-21 and GzmB in SLE and other AD. Moreover, our data suggest that IL-21 may have disease-modifying characteristics by inducing GzmB in CD5(+) B cells and by suppressing their expansion. Our results provide the rationale for further evaluation of the therapeutic potential of IL-21 in certain AD such as SLE.
- Subjects :
- Adolescent
Adult
Aged
B-Lymphocytes immunology
B-Lymphocytes pathology
CD5 Antigens biosynthesis
Cell Proliferation
Cell Survival immunology
Female
Granzymes genetics
Humans
Immune Tolerance
Interleukins blood
Interleukins genetics
Interleukins immunology
Lupus Erythematosus, Systemic blood
Male
Middle Aged
Receptor Cross-Talk immunology
Receptors, Antigen, B-Cell immunology
Receptors, Interleukin-21 biosynthesis
Receptors, Interleukin-21 genetics
B-Lymphocytes metabolism
Granzymes metabolism
Interleukins biosynthesis
Lupus Erythematosus, Systemic immunology
Receptors, Antigen, B-Cell metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 40
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20394077
- Full Text :
- https://doi.org/10.1002/eji.200940113