Rapid detection and identification of probiotic Lactobacillus casei ATCC 393 by multiplex PCR.

Authors :: Karapetsas A
Vavoulidis E
Galanis A
Sandaltzopoulos R
Kourkoutas Y
Source :: Journal of molecular microbiology and biotechnology [J Mol Microbiol Biotechnol] 2010; Vol. 18 (3), pp. 156-61. Date of Electronic Publication: 2010 Apr 13.
Publication Year :: 2010
Abstract: Many functional foods containing probiotic strains have been developed recently. Lactobacillus casei ATCC 393 is one of the most frequently used cultures in probiotic products. The present study aimed to develop a method for the detection and identification of L. casei ATCC 393 based on genetic polymorphisms of the hsp60 gene. A multiplex polymerase chain reaction (PCR) assay was designed, utilizing two novel strain-specific primer sets that enable identification of L. casei ATCC 393.The accuracy of our method was further confirmed by successful identification of our strain in probiotic cheese. The method described is an easy to use, rapid, inexpensive and accurate tool that may be readily applied to food, fecal and intestinal samples.<br /> (2010 S. Karger AG, Basel.)

Subjects :: Bacterial Proteins genetics
Chaperonin 60 genetics
Cheese microbiology
DNA Primers genetics
DNA, Bacterial genetics
Humans
Lacticaseibacillus casei genetics
Sensitivity and Specificity
Bacteriological Techniques methods
Lacticaseibacillus casei isolation & purification
Polymerase Chain Reaction methods
Probiotics

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