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Beta-carotene inhibits Helicobacter pylori-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 in human gastric epithelial AGS cells.
- Source :
-
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society [J Physiol Pharmacol] 2009 Dec; Vol. 60 Suppl 7, pp. 131-7. - Publication Year :
- 2009
-
Abstract
- Unlabelled: Reactive oxygen species (ROS) play critical roles in Helicobacter pylori (H. pylori)-associated gastric ulceration and carcinogenesis. Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) are involved in H. pylori-induced gastric diseases. Previously we demonstrated that H. pylori in Korean isolates induced the activation of mitogen-activated protein kinases (MAPK) and oxidant-sensitive transcription factors NF-kappaB and AP-1 which mediates the expression of iNOS and COX-2 in gastric epithelial AGS cells. beta-Carotene shows antioxidant activity and inhibits NF-kappaB-dependent gene expression in various cells. Present study aims to investigate whether beta-carotene inhibits H. pylori-induced expression of iNOS and COX-2 by suppressing the activation of MAPK, NF-kappaB, and AP-1 in gastric epithelial AGS cells. HP99 (H. pylori in Korean isolates) was added to AGS cells at the ratio of bacterium/cell, 300/1. beta-carotene inhibited H. pylori-induced increase in ROS level, the activation of MAPK (p38, the c-Jun NH2-terminal protein kinases, the extracellular signal-regulated kinases), NF-kappaB, and AP-1 and the expression of iNOS and COX-2 in AGS cells.<br />Conclusion: beta-carotene inhibits oxidant-mediated activation of inflammatory signaling and suppresses the expression of iNOS and COX-2 in gastric epithelial AGS cells infected with H. pylori.
- Subjects :
- Cell Line
Cyclooxygenase 2 genetics
DNA-Binding Proteins metabolism
Enzyme Activation
Epithelial Cells metabolism
Epithelial Cells pathology
Extracellular Signal-Regulated MAP Kinases metabolism
Gastric Mucosa microbiology
Gastric Mucosa pathology
Helicobacter Infections complications
Humans
I-kappa B Kinase metabolism
Inflammation Mediators metabolism
JNK Mitogen-Activated Protein Kinases metabolism
NF-kappa B metabolism
Nitric Oxide Synthase Type II genetics
Phosphorylation
RNA, Messenger metabolism
Reactive Oxygen Species metabolism
Stomach Ulcer enzymology
Stomach Ulcer metabolism
Stomach Ulcer microbiology
Transcription Factor AP-1 metabolism
p38 Mitogen-Activated Protein Kinases metabolism
Cyclooxygenase 2 metabolism
Gastric Mucosa metabolism
Gene Expression Regulation, Enzymologic
Helicobacter pylori physiology
Inflammation Mediators antagonists & inhibitors
Nitric Oxide Synthase Type II metabolism
beta Carotene pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1899-1505
- Volume :
- 60 Suppl 7
- Database :
- MEDLINE
- Journal :
- Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
- Publication Type :
- Academic Journal
- Accession number :
- 20388956