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Global analysis of quorum sensing targets in the intracellular pathogen Brucella melitensis 16 M.
- Source :
-
Journal of proteome research [J Proteome Res] 2010 Jun 04; Vol. 9 (6), pp. 3200-17. - Publication Year :
- 2010
-
Abstract
- Many pathogenic bacteria use a regulatory process termed quorum sensing (QS) to produce and detect small diffusible molecules to synchronize gene expression within a population. In Gram-negative bacteria, the detection of, and response to, these molecules depends on transcriptional regulators belonging to the LuxR family. Such a system has been discovered in the intracellular pathogen Brucella melitensis, a Gram-negative bacterium responsible for brucellosis, a worldwide zoonosis that remains a serious public health concern in countries were the disease is endemic. Genes encoding two LuxR-type regulators, VjbR and BabR, have been identified in the genome of B. melitensis 16 M. A DeltavjbR mutant is highly attenuated in all experimental models of infection tested, suggesting a crucial role for QS in the virulence of Brucella. At present, no function has been attributed to BabR. The experiments described in this report indicate that 5% of the genes in the B. melitensis 16 M genome are regulated by VjbR and/or BabR, suggesting that QS is a global regulatory system in this bacterium. The overlap between BabR and VjbR targets suggest a cross-talk between these two regulators. Our results also demonstrate that VjbR and BabR regulate many genes and/or proteins involved in stress response, metabolism, and virulence, including those potentially involved in the adaptation of Brucella to the oxidative, pH, and nutritional stresses encountered within the host. These findings highlight the involvement of QS as a major regulatory system in Brucella and lead us to suggest that this regulatory system could participate in the spatial and sequential adaptation of Brucella strains to the host environment.
- Subjects :
- 4-Butyrolactone analogs & derivatives
4-Butyrolactone metabolism
Bacterial Proteins chemistry
Bacterial Proteins classification
Brucella melitensis chemistry
Brucella melitensis metabolism
Chromatin Immunoprecipitation
Electrophoresis, Gel, Two-Dimensional
Gene Expression Profiling
Homoserine analogs & derivatives
Homoserine metabolism
Metabolic Networks and Pathways
Oxidative Stress physiology
Promoter Regions, Genetic
Proteome chemistry
Repressor Proteins chemistry
Repressor Proteins metabolism
Reproducibility of Results
Trans-Activators chemistry
Trans-Activators metabolism
Transcription Factors chemistry
Transcription Factors metabolism
Bacterial Proteins metabolism
Brucella melitensis physiology
Proteomics methods
Quorum Sensing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 9
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 20387905
- Full Text :
- https://doi.org/10.1021/pr100068p