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Culture-independent analysis of lactic acid bacteria diversity associated with mezcal fermentation.
- Source :
-
Current microbiology [Curr Microbiol] 2010 Nov; Vol. 61 (5), pp. 444-50. Date of Electronic Publication: 2010 Apr 10. - Publication Year :
- 2010
-
Abstract
- Mezcal is an alcoholic beverage obtained from the distillation of fermented juices of cooked Agave spp. plant stalks (agave must), and each region in Mexico with denomination of origin uses defined Agave species to prepare mezcal with unique organoleptic characteristics. During fermentation to produce mezcal in the state of Tamaulipas, not only alcohol-producing yeasts are involved, but also a lactic acid bacterial community that has not been characterized yet. In order to address this lack of knowledge on this traditional Mexican beverage, we performed a DGGE-16S rRNA analysis of the lactic acid bacterial diversity and metabolite accumulation during the fermentation of a typical agave must that is rustically produced in San Carlos County (Tamaulipas, Mexico). The analysis of metabolite production indicated a short but important malolactic fermentation stage not previously described for mezcal. The denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA genes showed a distinctive lactic acid bacterial community composed mainly of Pediococcus parvulus, Lactobacillus brevis, Lactobacillus composti, Lactobacillus parabuchneri, and Lactobacillus plantarum. Some atypical genera such as Weissella and Bacillus were also found in the residual must. Our results suggest that the lactic acid bacteria could strongly be implicated in the organoleptic attributes of this traditional Mexican distilled beverage.
- Subjects :
- Bacillus classification
Bacillus genetics
Bacillus metabolism
Base Sequence
DNA Fingerprinting
DNA, Ribosomal analysis
Denaturing Gradient Gel Electrophoresis
Fermentation
Food Microbiology
Lactobacillales classification
Lactobacillales genetics
Lactobacillales metabolism
Lactobacillus classification
Lactobacillus genetics
Lactobacillus isolation & purification
Lactobacillus metabolism
Levilactobacillus brevis classification
Levilactobacillus brevis genetics
Levilactobacillus brevis isolation & purification
Levilactobacillus brevis metabolism
Lactobacillus plantarum classification
Lactobacillus plantarum genetics
Lactobacillus plantarum isolation & purification
Lactobacillus plantarum metabolism
Mexico
Pediococcus classification
Pediococcus genetics
Pediococcus isolation & purification
Pediococcus metabolism
Phylogeny
Polymerase Chain Reaction
RNA, Bacterial genetics
RNA, Ribosomal, 16S analysis
Weissella classification
Weissella genetics
Weissella isolation & purification
Weissella metabolism
Agave microbiology
Alcoholic Beverages microbiology
Bacillus isolation & purification
Lactic Acid metabolism
Lactobacillales isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 61
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 20383505
- Full Text :
- https://doi.org/10.1007/s00284-010-9636-z