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A solvent-exposed patch in chaperone-bound YopE is required for translocation by the type III secretion system.
- Source :
-
Journal of bacteriology [J Bacteriol] 2010 Jun; Vol. 192 (12), pp. 3114-22. Date of Electronic Publication: 2010 Apr 09. - Publication Year :
- 2010
-
Abstract
- Most effector proteins of bacterial type III secretion (T3S) systems require chaperone proteins for translocation into host cells. Such effectors are bound by chaperones in a conserved and characteristic manner, with the chaperone-binding (Cb) region of the effector wound around the chaperone in a highly extended conformation. This conformation has been suggested to serve as a translocation signal in promoting the association between the chaperone-effector complex and a bacterial component required for translocation. We sought to test a prediction of this model by identifying a potential association site for the Yersinia pseudotuberculosis chaperone-effector pair SycE-YopE. We identified a set of residues in the YopE Cb region that are required for translocation but are dispensable for expression, SycE binding, secretion into the extrabacterial milieu, and stability in mammalian cells. These residues form a solvent-exposed patch on the surface of the chaperone-bound Cb region, and thus their effect on translocation is consistent with the structure of the chaperone-bound Cb region serving as a signal for translocation.
- Subjects :
- Amino Acid Sequence
Amino Acid Substitution
Bacterial Outer Membrane Proteins chemistry
Bacterial Outer Membrane Proteins genetics
Gene Expression Regulation, Bacterial physiology
HeLa Cells
Humans
Mutation
Protein Conformation
Bacterial Outer Membrane Proteins metabolism
Molecular Chaperones metabolism
Protein Transport physiology
Yersinia pseudotuberculosis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5530
- Volume :
- 192
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 20382763
- Full Text :
- https://doi.org/10.1128/JB.00113-10