Induction of apoptosis in densovirus infected Aedes aegypti mosquitoes.

Authors :: Roekring S
Smith DR
Source :: Journal of invertebrate pathology [J Invertebr Pathol] 2010 Jul; Vol. 104 (3), pp. 239-41. Date of Electronic Publication: 2010 Apr 09.
Publication Year :: 2010
Abstract: The mechanism of death in densovirus infected mosquitoes remains unexplored. This study investigated the cellular consequences of densovirus infection in Aedes aegypti mosquitoes after a second generation challenge with a densovirus isolated from adult Aedes albopictus mosquitoes in Thailand (AThDNV). Specimens were analyzed by TUNEL assay, fluorescent in situ hybridization (FISH) and a calorimic assay to detect activation of caspase 3-like activity. After challenge, moribund mosquitoes showed considerable evidence of TUNEL positive cells. The caspase 3-like activity assay showed that the presence of TUNEL positive cells was associated with increased levels of activated caspase 3-like activity in AThDNV infected mosquitoes.<br /> ((c) 2010 Elsevier Inc. All rights reserved.)