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Effects of high-dose major components in oral disinfectants on the cell cycle and apoptosis in primary human gingival fibroblasts in vitro.
- Source :
-
Dental materials journal [Dent Mater J] 2010 Jan; Vol. 29 (1), pp. 75-83. - Publication Year :
- 2010
-
Abstract
- We evaluated the effects of high-dose major components in oral disinfectants on oral cells from the standpoints of the cell cycle and apoptosis. We examined the viability and cell cycle of human gingival fibroblasts (HGFs) treated with the components of dental disinfectants, benzethonium chloride (BEC), benzalkonium chloride (BAC), and povidone iodine (PVD-I) using a cell counting kit and flow cytometry. The IC(50) inhibitory concentration value in HGF cultures at 24 hours was 1.3x10(-2) mM BEC, 6.0x10(-3) mM BAC, and 2.6x10(-1) mM PVD-I. In the cell cycle analysis, propidium iodide-stained HGFs were arrested in G(0)/G(1) of the cell cycle by all three disinfectants, and in the apoptosis assay, annexin V-FITC/PI-stained HGFs that became apoptotic at 5.0x10(-2) and 1.0x10(-1) mM BEC and 5.0x10(-2) and 1.0x10(-1) mM BAC, but not in PVD-I at concentrations as high as 5.0x10(-1) mM. Our findings describe the effects of high-dose oral disinfectants, rather than clinical concentrations. Nevertheless, appreciating the effects of high-dose disinfectants absorbed into the human body is important, where they may accumulate in specific tissues and cells.
- Subjects :
- Analysis of Variance
Benzalkonium Compounds toxicity
Benzethonium toxicity
Cells, Cultured
Chi-Square Distribution
Dose-Response Relationship, Drug
Fibroblasts drug effects
Flow Cytometry
Gingiva cytology
Humans
Inhibitory Concentration 50
Povidone-Iodine toxicity
Apoptosis
Cell Cycle drug effects
Dental Disinfectants toxicity
Gingiva drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0287-4547
- Volume :
- 29
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Dental materials journal
- Publication Type :
- Academic Journal
- Accession number :
- 20379016
- Full Text :
- https://doi.org/10.4012/dmj.2009-031