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Mouse neutrophil extracellular traps in microbial infections.
- Source :
-
Journal of innate immunity [J Innate Immun] 2009; Vol. 1 (3), pp. 181-93. Date of Electronic Publication: 2009 Feb 26. - Publication Year :
- 2009
-
Abstract
- Neutrophil extracellular traps (NETs) play an important role in innate immunity to microbial infections. NETs have been described in several species, but the molecular details of NET formation and their role in infection has not been addressed, partly because we lack optimal experimental models. Here we describe tools to investigate NET formation in neutrophils isolated from mice. Upon in vitro stimulation of wild-type mouse neutrophils with PMA, we analyzed 3 important steps in the process of NET formation: reactive oxygen species (ROS) production, NET cell death and NET release. As expected, neutrophils from NADPH oxidase-deficient mice failed to produce ROS and did not die nor release NETs upon stimulation. We found that neutrophils from several mouse strains produced NETs with different efficiency and that NET formation correlated with the amount of ROS produced. Activation with Candida albicans also resulted in ROS production and NET cell death. The hyphal form of this fungus induced NETs more effectively than the yeast form. With this work, we provide tools to study in vitro NET assembly in the mouse system.<br /> (Copyright 2009 S. Karger AG, Basel.)
- Subjects :
- Animals
Candidiasis microbiology
Cell Death
Cellular Structures metabolism
DNA metabolism
Disease Models, Animal
Immunity, Innate
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred Strains
Mice, Knockout
Microscopy, Electron, Scanning
NADPH Oxidases genetics
Proteins metabolism
Reactive Oxygen Species metabolism
Species Specificity
Tetradecanoylphorbol Acetate immunology
Tetradecanoylphorbol Acetate pharmacology
Candida albicans immunology
Candida albicans pathogenicity
Candidiasis immunology
Cellular Structures ultrastructure
Neutrophil Activation immunology
Neutrophils cytology
Neutrophils immunology
Neutrophils metabolism
Neutrophils ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1662-8128
- Volume :
- 1
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of innate immunity
- Publication Type :
- Academic Journal
- Accession number :
- 20375576
- Full Text :
- https://doi.org/10.1159/000205281