Back to Search
Start Over
Characterization of extended-spectrum beta-lactamases produced by Escherichia coli isolated from hospitalized and nonhospitalized patients: emergence of CTX-M-15-producing strains causing urinary tract infections.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2010 Jun; Vol. 16 (2), pp. 129-34. - Publication Year :
- 2010
-
Abstract
- Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates were obtained from hospitalized and nonhospitalized patients in Belgium between August 2006 and November 2007. The antimicrobial susceptibility of these isolates was determined and their ESBL genes were characterized. Clonal relationships between the CTX-M-producing E. coli isolates causing urinary tract infections were also studied. A total of 90 hospital- and 45 community-acquired cephalosporin-resistant E. coli isolates were obtained. Tetracycline, enrofloxacine, gentamicin, and trimethoprim-sulfamethaxozole resistance rates were significantly different between the community-onset and hospital-acquired isolates. A high diversity of different ESBLs was observed among the hospital-acquired E. coli isolates, whereas CTX-M-15 was dominating among the community-acquired E. coli isolates (n = 28). Thirteen different pulsed-field gel electrophoresis profiles were observed in the community-acquired CTX-M-15-producing E. coli, indicating that multiple clones have acquired the bla(CTX-M-15) gene. All community-acquired CTX-M-15-producing E. coli isolates of phylogroups B2 and D were assigned to the sequence type ST131. The hospital-acquired CTX-M-15-producing E. coli isolates of phylogroups B2, B1, A, and D corresponded to ST131, ST617, ST48, and ST405, respectively. In conclusion, CTX-M-type ESBLs have emerged as the predominant class of ESBLs produced by E. coli isolates in the hospital and community in Belgium. Of particular concern is the predominant presence of the CTX-M-15 enzyme in ST131 community-acquired E. coli.
- Subjects :
- Anti-Bacterial Agents pharmacology
Belgium epidemiology
Community-Acquired Infections microbiology
Cross Infection microbiology
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Escherichia coli enzymology
Escherichia coli genetics
Escherichia coli Infections epidemiology
Escherichia coli Infections microbiology
Escherichia coli Proteins biosynthesis
Escherichia coli Proteins genetics
Humans
Microbial Sensitivity Tests methods
Polymerase Chain Reaction
Urinary Tract Infections microbiology
beta-Lactam Resistance
beta-Lactamases genetics
Community-Acquired Infections epidemiology
Cross Infection epidemiology
Escherichia coli drug effects
Urinary Tract Infections epidemiology
beta-Lactamases biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 20370505
- Full Text :
- https://doi.org/10.1089/mdr.2009.0132