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Differential regulation of vasoactive intestinal peptide (VIP) in the dentate gyrus and hippocampus via the NO-cGMP pathway following kainic acid-induced seizure in the rat.
- Source :
-
Journal of molecular neuroscience : MN [J Mol Neurosci] 2010 Nov; Vol. 42 (3), pp. 359-69. Date of Electronic Publication: 2010 Apr 06. - Publication Year :
- 2010
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Abstract
- We have previously shown that kainic acid (KA) increases nitric oxide (NO) synthase (NOS) production in the rat dentate gyrus (DG) and hippocampus (CA3), and NOS inhibition [(by N(G)-nitro-L-arginine methylester (L-NAME)] modulates the vasoactive intestinal peptide (VIP)-responsive gene, activity-dependent neuroprotective protein, and alters neuro- and astrogliogenesis (Cosgrave et al. in Neurobiol Dis 30(3):281-292 2008, J Mol Neurosci 39(1-2):9-21, 2009, 2010). In the present study, using the same model we demonstrate that VIP synthesis is differentially regulated by the NO-cyclic guanosine monophosphate (cGMP) pathway in the DG and CA3 at 3 h and 3 days post-KA. At 3 h post-KA: In L-NAME+KA/7-nitroindazole (7-NI)+KA, stratum granulosum (SG) and subgranular zone (SGZ) cells were intensely stained for VIP when compared with L-NAME/7-NI/KA alone. Soluble guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, blocks cGMP production), suppressed astrocytic activation (glial fibrillary acidic protein) but other cell types were VIP(+); however, ODQ+KA suppressed overall VIP synthesis in the DG. At 3 days post-KA: In L-NAME+KA/7-NI+KA, SGZ and SG cells continued to express VIP, while in the KA alone, only SGZ cells were VIP(+). ODQ increased VIP(+) cells in the SG, and in contrast to 3 h, VIP-containing nNOS(+) cells increased in ODQ+KA when compared to vehicle+KA. In the hippocampus, 7-NI/ODQ had no effect on VIP at 3 h/3 days, while L-NAME+KA at 3 days increased VIP(+) cells, but reduced VIP-like immunoreactivity in astrocytes. These results suggest that the NO-cGMP pathway differentially regulates VIP in the DG and hippocampus during seizure.
- Subjects :
- Animals
Dentate Gyrus cytology
Dentate Gyrus metabolism
Enzyme Inhibitors pharmacology
Excitatory Amino Acid Agonists pharmacology
Guanylate Cyclase antagonists & inhibitors
Guanylate Cyclase metabolism
Hippocampus cytology
Hippocampus metabolism
Indazoles pharmacology
Male
NG-Nitroarginine Methyl Ester pharmacology
Nitric Oxide Synthase antagonists & inhibitors
Oxadiazoles pharmacology
Quinoxalines pharmacology
Rats
Rats, Wistar
Receptors, Cytoplasmic and Nuclear antagonists & inhibitors
Receptors, Cytoplasmic and Nuclear metabolism
Soluble Guanylyl Cyclase
Vasoactive Intestinal Peptide genetics
Cyclic GMP metabolism
Dentate Gyrus drug effects
Hippocampus drug effects
Kainic Acid pharmacology
Nitric Oxide metabolism
Signal Transduction physiology
Vasoactive Intestinal Peptide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1166
- Volume :
- 42
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular neuroscience : MN
- Publication Type :
- Academic Journal
- Accession number :
- 20369387
- Full Text :
- https://doi.org/10.1007/s12031-010-9353-x