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Chemokine levels in subretinal fluid obtained during scleral buckling surgery after rhegmatogenous retinal detachment.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2010 Aug; Vol. 51 (8), pp. 4143-50. Date of Electronic Publication: 2010 Mar 24. - Publication Year :
- 2010
-
Abstract
- Purpose: Interleukin (IL)-6, a multifunctional cytokine with regulatory functions in wound healing, and several chemokines have been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR) after rhegmatogenous retinal detachment (RRD). The exact role of these chemokines, their correlation with IL-6 after primary RRD, and their association with the future development of PVR are not yet known.<br />Methods: A multiplex immunoassay was used to determine levels of 15 different chemokines and IL-6 in subretinal fluid samples obtained during scleral buckling surgery for primary RRD. Samples from patients with preoperative uveitis, preoperative trauma, or preoperative vitreous hemorrhage were excluded. Patients who developed a redetachment due to postsurgical PVR within 2.5 months (n = 21) were compared with control subjects who had an uncomplicated retinal detachment during the overall follow-up period (n = 54). Control subjects were matched for sex, age, and storage time.<br />Results: Levels of IL-6 (P = 0.001), MIF (P = 0.016), CCL2 (P = 0.041), CCL11 (P = 0.012), CCL17 (P = 0.003), CCL18 (P = 0.007), CCL19 (P < 0.001), CCL22 (P < 0.001), CXCL8 (P = 0.027), CXCL9 (P = 0.007), and CXCL10 (P = 0.002) were significantly higher in patients who developed postoperative PVR after primary RRD than in patients with uncomplicated retinal detachment. A significant positive correlation was observed between IL-6 and both CCL22 (r = 0.538; P < 0.0001) and CXCL8 (r = 0.645; P < 0.0001).<br />Conclusions: Various chemokines and IL-6 are upregulated in patients in whom fibrotic membranes develop after primary RRD repair and may therefore be involved in the future development of postoperative PVR.
- Subjects :
- Adult
Aged
Exudates and Transudates metabolism
Female
Humans
Immunoassay
Interleukin-6 metabolism
Male
Middle Aged
Recurrence
Retinal Detachment metabolism
Up-Regulation
Vitreoretinopathy, Proliferative etiology
Body Fluids metabolism
Chemokines metabolism
Postoperative Complications
Retinal Detachment surgery
Scleral Buckling
Vitreoretinopathy, Proliferative metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 51
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 20335622
- Full Text :
- https://doi.org/10.1167/iovs.09-5057