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Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine.
- Source :
-
Journal of bacteriology [J Bacteriol] 2010 Jun; Vol. 192 (11), pp. 2722-8. Date of Electronic Publication: 2010 Mar 19. - Publication Year :
- 2010
-
Abstract
- Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and energy sources but also enhance synthesis of the cell signaling molecule Pseudomonas quinolone signal (PQS). The present study investigates the role of the aromatic amino acid-responsive regulator PhhR in mediating these phenotypes. Transcriptome analysis revealed that PhhR controls four putative transcriptional units (phhA, hpd, hmgA, and dhcA) involved in aromatic amino acid catabolism; however, genes involved in PQS biosynthesis were unaffected. The phhA, hpd, hmgA, and dhcA promoters were mapped by primer extension, and purified His(6)-PhhR was shown to bind the phhA, hpd, and dhcA promoters in vitro by use of electrophoretic mobility shift assays. Our work characterizes a transcriptional regulator of catabolic genes induced during P. aeruginosa growth in CF sputum.
- Subjects :
- Bacterial Proteins genetics
Base Sequence
Electrophoretic Mobility Shift Assay
Gene Expression Profiling
Gene Expression Regulation, Bacterial drug effects
Gene Expression Regulation, Bacterial genetics
Genetic Complementation Test
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Promoter Regions, Genetic
Protein Binding genetics
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa metabolism
Trans-Activators genetics
Bacterial Proteins metabolism
Phenylalanine pharmacology
Pseudomonas aeruginosa genetics
Trans-Activators metabolism
Tyrosine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5530
- Volume :
- 192
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 20304990
- Full Text :
- https://doi.org/10.1128/JB.00112-10