Back to Search
Start Over
Immunoblotting human C4 bound to human erythrocytes in vivo and in vitro.
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 1991 May; Vol. 84 (2), pp. 263-9. - Publication Year :
- 1991
-
Abstract
- A study of C4 bound to human erythrocytes in vitro and in vivo has been made by immunoblotting with mouse monoclonal anti-C4c and anti-C4d and human polyclonal anti-C4d (Rodgers and Chido) following SDS-PAGE. Multi-banded patterns differentiated between C4A and C4B isotypes. Treatment of EC4b with trypsin eliminated immunoblotting but not agglutination reactions. Serum inactivation (factor I) of EC4b resulted in banding patterns similar to those obtained from patients' EC4d. Treatment of EC4b membranes with NH2OH affected many of the bands, two were lost, one was markedly reduced and others had altered SDS-PAGE mobility. Interpretation of the bands has been made in terms of C4-acceptor complexes and inactivation fragments of C4. A distinct difference in the banding of C4A and C4B isotypes has been detected.
- Subjects :
- Ammonium Hydroxide
Blotting, Western
Chloroquine pharmacology
Chymotrypsin pharmacology
Complement C4a drug effects
Complement C4b drug effects
Dithiothreitol pharmacology
Electrophoresis, Polyacrylamide Gel
Hemagglutination Tests
Humans
Hydroxides pharmacology
Hydroxylamine
Hydroxylamines pharmacology
Radioligand Assay
Trypsin pharmacology
beta-Aminoethyl Isothiourea pharmacology
Complement C4 analysis
Erythrocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9104
- Volume :
- 84
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2025953
- Full Text :
- https://doi.org/10.1111/j.1365-2249.1991.tb08159.x