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Nocardiopsis terrae sp. nov., a halophilic actinomycete isolated from saline soil.
- Source :
-
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2010 Jun; Vol. 98 (1), pp. 31-8. Date of Electronic Publication: 2010 Mar 17. - Publication Year :
- 2010
-
Abstract
- A Gram-positive, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic, filamentous actinomycete strain, designated YIM 90022(T), was isolated from saline soil collected from the Qaidam Basin, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus Nocardiopsis and the sequence similarities between the isolate and the type strains of members of the genus Nocardiopsis were in the range of 95.1-98.7%. Phenotypic and chemotaxonomic properties of this organism also indicated that strain YIM 90022(T) was a member of the genus Nocardiopsis. The strain grew well on most of the media tested, producing yellow-white to deep brown substrate mycelium and white aerial mycelium. Light gray to deep brown diffusible pigments were produced. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores on them. The strain grew in the presence of 1-15% (w/v) total salts (optimum, 3-5%) and at pH 6.0-10.5 (optimum, pH 8.5) and 10-45 degrees C (optimum, 30 degrees C). Whole-cell hydrolysates of strain YIM 90022(T) contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H(4)), MK-9(H(8)), MK-10(H(6)) and MK-10(H(8)). Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylmethylethanolamine. The major cellular fatty acids were iso-C(16:0), anteiso-C(17:0), 10-methyl-C(18:0) and 10-methyl-C(17:0). The DNA G + C content of strain YIM 90022(T) was 71.5 mol%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain YIM 90022(T) represents a new species of the genus Nocardiopsis, for which the name Nocardiopsis terrae sp. nov. is proposed. The type strain is YIM 90022(T) (=CCTCC AA 208011(T) =KCTC 19431(T)).
- Subjects :
- Actinomycetales genetics
Actinomycetales physiology
Bacterial Typing Techniques
Base Composition
China
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fatty Acids analysis
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S genetics
Salinity
Sequence Analysis, DNA
Actinomycetales classification
Actinomycetales isolation & purification
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1572-9699
- Volume :
- 98
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 20232249
- Full Text :
- https://doi.org/10.1007/s10482-010-9425-5