A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet.

Authors :: Cong WT
Zhu ZX
He HZ
Jin Y
Jiang CX
Choi JK
Jin LT
Li XK
Source :: Analytical biochemistry [Anal Biochem] 2010 Jul 01; Vol. 402 (1), pp. 99-101. Date of Electronic Publication: 2010 Mar 15.
Publication Year :: 2010
Abstract: We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.<br /> (2010 Elsevier Inc. All rights reserved.)

Subjects :: Sensitivity and Specificity
Staining and Labeling economics
Viral Proteins genetics
DNA analysis
Electrophoresis, Polyacrylamide Gel methods
Rosaniline Dyes
Staining and Labeling methods

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