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Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2010 May; Vol. 67 (1), pp. 22-9. Date of Electronic Publication: 2010 Mar 12. - Publication Year :
- 2010
-
Abstract
- Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Research Triangle Park, NC) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but 3 times faster and offers an inexpensive and convenient diagnostic test for microbial DNA.<br /> ((c) 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Automation
Community-Acquired Infections microbiology
Humans
Mycoplasma pneumoniae genetics
Nasopharynx microbiology
Pneumonia, Mycoplasma microbiology
Point-of-Care Systems
Sensitivity and Specificity
Time Factors
Bacteriological Techniques methods
Microfluidics methods
Molecular Diagnostic Techniques methods
Mycoplasma pneumoniae isolation & purification
Pneumonia, Mycoplasma diagnosis
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 67
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 20227222
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2009.12.020