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A comparison of the autohydrolysis and ammonia fiber explosion (AFEX) pretreatments on the subsequent enzymatic hydrolysis of coastal Bermuda grass.
- Source :
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Bioresource technology [Bioresour Technol] 2010 Jul; Vol. 101 (14), pp. 5449-58. Date of Electronic Publication: 2010 Mar 12. - Publication Year :
- 2010
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Abstract
- Two distinct pretreatment technologies, autohydrolysis and AFEX, have been applied to coastal Bermuda grass (CBG) followed by enzymatic hydrolysis in order to compare the effects of pretreatment on the subsequent sugar generation. Furthermore, the influence of structural features from each pretreatment on biomass digestibility was characterized with SEM, ATR-FTIR, and XRD. Enzymatic conversion of pretreated solids from the pretreatments increased with elevated temperature and longer residence times. AFEX pretreatment at 100 degrees C for 30 min produced a sugar yield of 94.8% of theoretical possible with 30 FPU/g enzymatic loading, the maximum achieved with AFEX. It was also shown that with autohydrolysis at 170 degrees C for 60 min that 55.4% sugar yield of the theoretical possible was produced with a 30 FPU/g enzymatic loading, the maximum with autohydrolysis. AFEX pretreatment does not change the chemical composition of CBG but autohydrolysis reduces hemicellulose content in the pretreated solids. Both pretreatments cause re-localization of lignin components. There was no observed correlation between crystallinity and enzyme digestibility of the pretreated solids. AFEX pretreatment developed more enzymatic accessibility to pretreated solids of CBG than did autohydrolysis pretreatment, leading to more sugar generation through the whole process. The total amount of sugars accounted for with autohydrolysis decreases with increasing temperature, consistent with increased byproduct generation via thermal degradation reactions.<br /> (Published by Elsevier Ltd.)
- Subjects :
- Ammonia chemistry
Biotechnology methods
Crystallization
Ethanol chemistry
Fermentation
Microscopy, Electron, Scanning methods
Polysaccharides chemistry
Spectroscopy, Fourier Transform Infrared methods
Surface Properties
Temperature
Time Factors
X-Ray Diffraction methods
Cynodon metabolism
Hydrolysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 101
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 20223654
- Full Text :
- https://doi.org/10.1016/j.biortech.2010.02.055